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Caspase 3 anticorps (AA 29-175) (FITC)

L’anticorps Lapin Polyclonal anti-Caspase 3 a été validé pour WB, IHC, ICC et IF. Il convient pour détecter Caspase 3 dans des échantillons de Rat. Il y a 1 publication disponible.
N° du produit ABIN1858244

Aperçu rapide pour Caspase 3 anticorps (AA 29-175) (FITC) (ABIN1858244)

Antigène

Voir toutes Caspase 3 (CASP3) Anticorps
Caspase 3 (CASP3)

Reactivité

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Rat

Hôte

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Lapin

Clonalité

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Polyclonal

Conjugué

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Cet anticorp Caspase 3 est conjugé à/à la FITC

Application

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Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
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    AA 29-175

    Fonction

    FITC-Linked Polyclonal Antibody to Caspase 3 (CASP3)

    Séquence

    MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGLCIII NNKNFHKSTGMSARNGTDVD AANLRETFMA LKYEVRNKND LTREEIMELM DSVSKEDHSK RSSFVCVILS HGDEGVIFGT NGPVDLKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD

    Specificité

    The antibody is a rabbit polyclonal antibody raised against CASP3. It has been selected for its ability to recognize CASP3 in immunohistochemical staining and western blotting.

     Réactivité croisée

    Humain, Souris

    Purification

    Antigen-specific affinity chromatography followed by Protein A affinity chromatography

    Immunogène

    Recombinant CASP3 expressed in E.coli.
    The antibody is a rabbit polyclonal antibody raised against CASP3 conjugated to fitc.

    Isotype

    IgG
  • Indications d'application

    Western blotting: 0.2-2 μg/mL,1:250-2500 Immunohistochemistry: 5-20 μg/mL,1:25-100 Immunocytochemistry: 5-20 μg/mL,1:25-100 Optimal working dilutions must be determined by end user.

    Commentaires

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    500 μg/mL

    Buffer

    PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

    Agent conservateur

    Sodium azide

    Précaution d'utilisation

    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

    Conseil sur la manipulation

    Avoid repeated freeze/thaw cycles

    Stock

    4 °C,-20 °C

    Stockage commentaire

    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

    Date de péremption

    12 months
  • Chen, Cao, Asare, Lv, Zhu, Li, Wei, Gao, Zhang, Mao, Gao, Fan: "Amelioration of cardiac dysfunction and ventricular remodeling after myocardial infarction by danhong injection are critically contributed by anti-TGF-β-mediated fibrosis and angiogenesis mechanisms." dans: Journal of ethnopharmacology, Vol. 194, pp. 559-570, (2016) (PubMed).

  • Antigène

    Caspase 3 (CASP3)

    Autre désignation

    Caspase 3

    Sujet

    CPP32, CPP32B, SCA1, Apoptain, Yama, Apoptosis-Related Cysteine Peptidase, Cysteinyl Aspartate Specific Proteinases 3, SREBP cleavage activity 1

    Pathways

    Apoptose, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
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